lnvestigation Of Antibody Dependent Cell Mediated Cytotoxicitiy Levels in Myasthenia Gravis
Serap ÖZER1, Berril DÖNMEZ2, Özden ÜLKER3, Murat SAYAN1, Hakkı BAHAR1, Ahmet GENÇ2, Egemen İDİMAN2
1Dokuz Eylül Üniversitesi Tıp Fakültesi Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı
2Dokuz Eylül Üniversitesi Tıp Fakültesi Nöroloji Anabilim Dalı
3Dokuz Eylül Üniversitesi Tıp Fakültesi Nükleer Tıp Anabilim Dalı
Keywords: Antibody dependent cell mediated cytotoxicity, acetylcholine receptor antibody, myasthenia gravis.
Abstract
Myasthenia gravis (MG) is known as prototype disorder of humoral immunity mediatedby autoantibodies. A limited number of studies investigating the role of antibody dependent cell mediated cytotoxitity (ADCC) in pathogenesis of MG have contradictory results. In this study, the level of ADCC were measured in 30 patients with MG and 20 healthy individuals. The 18 hours 51Cr releasing test was used for measuring ADCC,effector cells were supplied from mononucleer cells of healthy individuals and target cells were suplied from the cells obtained from embrional rhabdomyosarcom cell culture. The patients had significantly higher levels of ADCC (mean: 43.9 %) compared to control group (mean: 27.6 %) (p<0.05). The patients with acethylcholine receptor antibodies(mean: 57.4 %) had significantly higher levels of ADCC compared to the patients withouth acethylcholine receptor antibodies (mean: 32.1 %) (p<0.01 ). ADCC levels were not effected by clinical status, tymectomy procedure, steroid and/or azathiopirin administration. In concusion, ADCC might have a role in immunological mechanisms inMG, and AChR antibodies might have an effect on ADCC.