Effect of Caffeic Acid Phenethyl Ester on Cerebellar Tissue Damage Secondary to Methanol Intoxication:  Experimental Study

Adalet Arıkanoğlu; Hatice Yüksel; Cüneyt Göçmez; Ertuğrul Uzar; Abdullah Acar; Mehmet Ufuk Aluçlu

doi:10.4274/Tnd.21939

Adalet Arıkanoğlu¹, Hatice Yüksel², Cüneyt Göçmez³, Ertuğrul Uzar¹, Abdullah Acar¹, Mehmet Ufuk Aluçlu¹

¹Dicle University Faculty Of Medicine, Department Of Neurology,diyarbakir
²Dicle University Faculty Of Medicine,department Of Medical Biochemistry, Diyarbakir
³Dicle University Faculty Of Medicine,department Of Neurosurgery,diyarbakir

Keywords: Cerebellum, oxidative stress, methanol, ethanol, CAPE

Abstract

OBJECTIVE: Previous studies have shown the role of oxidative stress in methanol neurotoxicity. CAPE is noted to have an antioxidant property by many experimental studies. In this study, we aim to investigate whether CAPE has a protective effect against oxidative stress observed in the cerebellar tissue in methanol intoxication

METHODS: In this study, a total of 40 rats were split into 5 groups: Control group (n=8), MTX-alone group (n=8), MTX+Methanol group (n=8), MTX+Methanol+Ethanol group (ie., ethanol group) (n=8), and MTX+Metanol+CAPE group (ie.,CAPE group) (n=8). All the rats except the control group were delivered methotrexate (MTX) therapy (0.3 mg/kg/day, via i.p. route) for 7 days in order to induce methanol toxicity. The control group received no drug therapy. Seven days later, 3 g/kg (i.p.) methanol was delivered in the ethanol and CAPE groups. Four hours after the delivery of methanol, ethanol group received 0.5 g/kg ethanol (i.p.) and CAPE group received 10 µmol/kg CAPE (i.p.), while the other groups were delivered only saline (i.p.). The rats were decapitated at 8 hours and the cerebellar tissues were removed. PON-1, TAS, and MDA levels were measured in the tissues.

RESULTS: MTX-alone group demonstrated decreased TAS and PON-1 levels (p=0.001 and p=0.004, respectively) and increased MDA level (p=0.001), as compared to the Control group. When MTX+Methanol group was compared with the MTX-alone group, MTX+Methanol group was found to have decreased TAS and PON-1 activities (p=0.037 and p=0.046, respectively) and increased MDA level (p=0.022). The Ethanol group was found to show a significant decrease in MDA level (p=0.001), as compared with the MTX+Methanol group. The CAPE group exhibited increased TAS and PON-1 levels (p=0.001 and p=0.001, respectively) and decreased MDA level, as compared with the MTX+Methanol group.

CONCLUSION: Cerebellum demonstrates oxidative stress secondary to methanol intoxication. CAPE therapy is more effective against cerebellar oxidative stress than ethanol therapy.