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Volume : 23 Issue : 3 Year : 2017

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Flow Cytometry Analysis of Peripheral Blood B cell Distribution of Multiple Sclerosis Patients [Turk J Neurol]
Turk J Neurol. Ahead of Print: TJN-87523

Flow Cytometry Analysis of Peripheral Blood B cell Distribution of Multiple Sclerosis Patients

Vuslat Yılmaz1, Deniz Ak Tura2, Canan Ulusoy1, Duygu Ozkan Yasargun2, Suzan Adin Cinar3, Recai Türkoğlu2
1Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Neuroscience, Istanbul, Turkey
2Haydarpasa Numune Education and Research Hospital, Department of Neurology, Istanbul, Turkey
3Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology, Istanbul, Turkey

Objective: Multiple sclerosis (MS) is a central nervous system (CNS) disease characterized by autoimmune inflammation and neurodegeneration. Damage to the CNS is thought to be mediated predominantly by activated pro-inflammatory T cells and antibody secreting B cells. A strong evidence of B cell functions in MS pathogenesis has come from trials of B cell depleting treatment. In this study, the peripheral blood frequencies of B cell subsets were measured by flow cytometry in patients to determine the disease specific B cell differences that might be associated with evolution to progressive forms of MS.
Materials and Methods: Peripheral blood mononuclear cells were separated from patients and healthy controls. Cells were stained with anti-human monoclonal antibodies (CD19-APC, CD27-FITC, IgD-APC/Cy7, CD138-PE, CD24-PerCP and CD38-Alexa fluor 700) and analyzed by flow cytometry.
Results: There were no significant differences between MS group and healthy controls by means of peripheral blood frequencies of B cells, immature, naïve, classical memory, plasma, plasmablasts and regulatory B cells. Only higher naïve B cell frequency tendency was determined in RRMS patients as compared to SPMS patients and healthy controls.
Conclusion: Peripheral blood B cell subset measurements are not likely to be used as a biomarker for prediction of disease progression. Although B cells have a well-known pathogenic significance, B cell population alterations do not occur during the progression of the disease.

Keywords: Multiple sclerosis, B cell, peripheral blood




Corresponding Author: Vuslat Yılmaz, Türkiye


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